human ppm1f rna-sequencing tissue data Search Results


phosphorylated nf κb p65 ser536  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phosphorylated nf κb p65 ser536
circPPM1F promotes M1 macrophage activation by <t>enhancing</t> <t>NF-κB</t> signaling. A. The location of circPPM1F in the PPM1F transcript was validated by Sanger sequencing. B. Total RNA was digested with or without RNase R, followed by quantitative real-time PCR (qRT-PCR) measurements of circPPM1F , PPM1F , MALAT1 , and GAPDH . C. The stability of circPPM1F was detected by qRT-PCR in THP1 macrophages after actinomycin D (Act D) treatment. D. qRT-PCR analysis of circPPM1F expression levels in THP1 macrophages following circPPM1F knockdown by two distinct siRNAs. E. qRT-PCR analyses of IL‐1β, TNF‐α, and CXCL10 in THP1 macrophages with conditional treatment. Mock-, untransfected, and unstimulated cells; mock+, LPS stimulated alone cells; si-scramble, LPS-stimulated cells following transfection with si-scramble; si-circPPM1F-2, LPS-stimulated cells following transfection with si-circPPM1F-2. F. ELISA analyses of secreted cytokine levels in THP1 macrophages with circPPM1F knockdown, followed by LPS treatment. G. qRT-PCR analyses of circPPM1F in THP1 macrophages with 3D5- circPPM1F or pZW1 transfection (left); M1-associated gene expressions in LPS stimulated THP1 macrophages overexpressing circPPM1F were quantified by qRT-PCR analysis (right). H. ELISA analyses of secreted cytokine levels in circPPM1F -overexpressed THP1 macrophages, followed by LPS treatment. I. Western blot showing total <t>p65,</t> p38, ERK1/2, JNK and their phosphorylation levels in THP1 macrophages with or without circPPM1F knockdown after LPS treatment. J. Western blotting analysis to evaluate levels of total p65, phosphorylated p65 in circPPM1F -overexpressed THP1 macrophages. The levels of p-p65 were normalized to that of β-tubulin and quantified using Image J software. Data are presented as mean ± SEM from three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ns indicates no significance.
Phosphorylated Nf κb P65 Ser536, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shrna dnm2 against exon potential off-target in mouse and dnm2  (ACGT Inc)
90
ACGT Inc shrna dnm2 against exon potential off-target in mouse and dnm2
circPPM1F promotes M1 macrophage activation by <t>enhancing</t> <t>NF-κB</t> signaling. A. The location of circPPM1F in the PPM1F transcript was validated by Sanger sequencing. B. Total RNA was digested with or without RNase R, followed by quantitative real-time PCR (qRT-PCR) measurements of circPPM1F , PPM1F , MALAT1 , and GAPDH . C. The stability of circPPM1F was detected by qRT-PCR in THP1 macrophages after actinomycin D (Act D) treatment. D. qRT-PCR analysis of circPPM1F expression levels in THP1 macrophages following circPPM1F knockdown by two distinct siRNAs. E. qRT-PCR analyses of IL‐1β, TNF‐α, and CXCL10 in THP1 macrophages with conditional treatment. Mock-, untransfected, and unstimulated cells; mock+, LPS stimulated alone cells; si-scramble, LPS-stimulated cells following transfection with si-scramble; si-circPPM1F-2, LPS-stimulated cells following transfection with si-circPPM1F-2. F. ELISA analyses of secreted cytokine levels in THP1 macrophages with circPPM1F knockdown, followed by LPS treatment. G. qRT-PCR analyses of circPPM1F in THP1 macrophages with 3D5- circPPM1F or pZW1 transfection (left); M1-associated gene expressions in LPS stimulated THP1 macrophages overexpressing circPPM1F were quantified by qRT-PCR analysis (right). H. ELISA analyses of secreted cytokine levels in circPPM1F -overexpressed THP1 macrophages, followed by LPS treatment. I. Western blot showing total <t>p65,</t> p38, ERK1/2, JNK and their phosphorylation levels in THP1 macrophages with or without circPPM1F knockdown after LPS treatment. J. Western blotting analysis to evaluate levels of total p65, phosphorylated p65 in circPPM1F -overexpressed THP1 macrophages. The levels of p-p65 were normalized to that of β-tubulin and quantified using Image J software. Data are presented as mean ± SEM from three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ns indicates no significance.
Shrna Dnm2 Against Exon Potential Off Target In Mouse And Dnm2, supplied by ACGT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/shrna dnm2 against exon potential off-target in mouse and dnm2/product/ACGT Inc
Average 90 stars, based on 1 article reviews
shrna dnm2 against exon potential off-target in mouse and dnm2 - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


circPPM1F promotes M1 macrophage activation by enhancing NF-κB signaling. A. The location of circPPM1F in the PPM1F transcript was validated by Sanger sequencing. B. Total RNA was digested with or without RNase R, followed by quantitative real-time PCR (qRT-PCR) measurements of circPPM1F , PPM1F , MALAT1 , and GAPDH . C. The stability of circPPM1F was detected by qRT-PCR in THP1 macrophages after actinomycin D (Act D) treatment. D. qRT-PCR analysis of circPPM1F expression levels in THP1 macrophages following circPPM1F knockdown by two distinct siRNAs. E. qRT-PCR analyses of IL‐1β, TNF‐α, and CXCL10 in THP1 macrophages with conditional treatment. Mock-, untransfected, and unstimulated cells; mock+, LPS stimulated alone cells; si-scramble, LPS-stimulated cells following transfection with si-scramble; si-circPPM1F-2, LPS-stimulated cells following transfection with si-circPPM1F-2. F. ELISA analyses of secreted cytokine levels in THP1 macrophages with circPPM1F knockdown, followed by LPS treatment. G. qRT-PCR analyses of circPPM1F in THP1 macrophages with 3D5- circPPM1F or pZW1 transfection (left); M1-associated gene expressions in LPS stimulated THP1 macrophages overexpressing circPPM1F were quantified by qRT-PCR analysis (right). H. ELISA analyses of secreted cytokine levels in circPPM1F -overexpressed THP1 macrophages, followed by LPS treatment. I. Western blot showing total p65, p38, ERK1/2, JNK and their phosphorylation levels in THP1 macrophages with or without circPPM1F knockdown after LPS treatment. J. Western blotting analysis to evaluate levels of total p65, phosphorylated p65 in circPPM1F -overexpressed THP1 macrophages. The levels of p-p65 were normalized to that of β-tubulin and quantified using Image J software. Data are presented as mean ± SEM from three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ns indicates no significance.

Journal: Theranostics

Article Title: Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus

doi: 10.7150/thno.48264

Figure Lengend Snippet: circPPM1F promotes M1 macrophage activation by enhancing NF-κB signaling. A. The location of circPPM1F in the PPM1F transcript was validated by Sanger sequencing. B. Total RNA was digested with or without RNase R, followed by quantitative real-time PCR (qRT-PCR) measurements of circPPM1F , PPM1F , MALAT1 , and GAPDH . C. The stability of circPPM1F was detected by qRT-PCR in THP1 macrophages after actinomycin D (Act D) treatment. D. qRT-PCR analysis of circPPM1F expression levels in THP1 macrophages following circPPM1F knockdown by two distinct siRNAs. E. qRT-PCR analyses of IL‐1β, TNF‐α, and CXCL10 in THP1 macrophages with conditional treatment. Mock-, untransfected, and unstimulated cells; mock+, LPS stimulated alone cells; si-scramble, LPS-stimulated cells following transfection with si-scramble; si-circPPM1F-2, LPS-stimulated cells following transfection with si-circPPM1F-2. F. ELISA analyses of secreted cytokine levels in THP1 macrophages with circPPM1F knockdown, followed by LPS treatment. G. qRT-PCR analyses of circPPM1F in THP1 macrophages with 3D5- circPPM1F or pZW1 transfection (left); M1-associated gene expressions in LPS stimulated THP1 macrophages overexpressing circPPM1F were quantified by qRT-PCR analysis (right). H. ELISA analyses of secreted cytokine levels in circPPM1F -overexpressed THP1 macrophages, followed by LPS treatment. I. Western blot showing total p65, p38, ERK1/2, JNK and their phosphorylation levels in THP1 macrophages with or without circPPM1F knockdown after LPS treatment. J. Western blotting analysis to evaluate levels of total p65, phosphorylated p65 in circPPM1F -overexpressed THP1 macrophages. The levels of p-p65 were normalized to that of β-tubulin and quantified using Image J software. Data are presented as mean ± SEM from three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ns indicates no significance.

Article Snippet: Lysates were resolved by electrophoresis, transferred to polyvinylidene fluoride (PVDF) membranes, and probed with antibodies directed against PPM1F (Abcam); HuR, phosphorylated NF-κB p65 (Ser536) (p-p65), p65, phosphorylated p38 (T180/Y182) (p-p38), p38, phosphorylated JNK (T183/Y185) (p-JNK), JNK, phosphorylated ERK1/2 (T202/Y204) (p-ERK1/2), ERK1/2, phosphorylated m-TOR (Ser2448) (p-mTOR), mTOR, phosphorylated Stat3 (Y705) (p-Stat3), Stat3, Bcl2 (Cell Signaling Technology, USA); Bax (EMD Millipore, USA), and β-tubulin (Abcam, USA).

Techniques: Activation Assay, Sequencing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Knockdown, Transfection, Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics, Software

circPPM1F competitively binds to HuR to impair PPM1F translation. A. Western blotting analysis to evaluate levels of PPM1F in circPPM1F knocked down-THP1 macrophages. B. qRT-PCR analyses of PPM1F expression in THP1 macrophages with circPPM1F knockdown. C. Analysis of PPM1F expression in THP1 macrophages transfected with PPM1F siRNA (200 nM) or control siRNA by qRT-PCR. D. Western blot analysis of PPM1F, p65, and p-p65 in THP1 macrophages with PPM1F knockdown, followed by LPS stimulation. E. Quantitative real-time PCR (qRT-PCR) analysis of M1-associated gene expression in THP1 macrophages with PPM1F knockdown, followed by LPS stimulation. F. qRT-PCR results showing the distribution of circPPM1F in the cytoplasmic and nuclear fractions of THP1 macrophages. GAPDH as cytoplasm control transcript, and U1 as nuclear control transcript. G. Putative HuR binding sites within circPPM1F full-length sequence. H. The enrichment levels of circPPM1F and PPM1F in the products of the RNA immunoprecipitation (RIP) assay (HuR IP compared with IgG IP) as detected by qRT-PCR. I. qRT-PCR analysis of HuR in THP1 macrophages transfected with HuR siRNA (200 nM) or control siRNA. J. Protein levels of PPM1F and HuR were detected by western blotting in THP1 macrophages with HuR knockdown. K. qRT-PCR analysis of circPPM1F and PPM1F in THP1 macrophages with HuR knockdown. The levels of PPM1F, p-p65 and HuR were normalized to those of β-tubulin and quantified using Image J software. Data are presented as mean ± SEM from three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ns indicates no significance.

Journal: Theranostics

Article Title: Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus

doi: 10.7150/thno.48264

Figure Lengend Snippet: circPPM1F competitively binds to HuR to impair PPM1F translation. A. Western blotting analysis to evaluate levels of PPM1F in circPPM1F knocked down-THP1 macrophages. B. qRT-PCR analyses of PPM1F expression in THP1 macrophages with circPPM1F knockdown. C. Analysis of PPM1F expression in THP1 macrophages transfected with PPM1F siRNA (200 nM) or control siRNA by qRT-PCR. D. Western blot analysis of PPM1F, p65, and p-p65 in THP1 macrophages with PPM1F knockdown, followed by LPS stimulation. E. Quantitative real-time PCR (qRT-PCR) analysis of M1-associated gene expression in THP1 macrophages with PPM1F knockdown, followed by LPS stimulation. F. qRT-PCR results showing the distribution of circPPM1F in the cytoplasmic and nuclear fractions of THP1 macrophages. GAPDH as cytoplasm control transcript, and U1 as nuclear control transcript. G. Putative HuR binding sites within circPPM1F full-length sequence. H. The enrichment levels of circPPM1F and PPM1F in the products of the RNA immunoprecipitation (RIP) assay (HuR IP compared with IgG IP) as detected by qRT-PCR. I. qRT-PCR analysis of HuR in THP1 macrophages transfected with HuR siRNA (200 nM) or control siRNA. J. Protein levels of PPM1F and HuR were detected by western blotting in THP1 macrophages with HuR knockdown. K. qRT-PCR analysis of circPPM1F and PPM1F in THP1 macrophages with HuR knockdown. The levels of PPM1F, p-p65 and HuR were normalized to those of β-tubulin and quantified using Image J software. Data are presented as mean ± SEM from three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ns indicates no significance.

Article Snippet: Lysates were resolved by electrophoresis, transferred to polyvinylidene fluoride (PVDF) membranes, and probed with antibodies directed against PPM1F (Abcam); HuR, phosphorylated NF-κB p65 (Ser536) (p-p65), p65, phosphorylated p38 (T180/Y182) (p-p38), p38, phosphorylated JNK (T183/Y185) (p-JNK), JNK, phosphorylated ERK1/2 (T202/Y204) (p-ERK1/2), ERK1/2, phosphorylated m-TOR (Ser2448) (p-mTOR), mTOR, phosphorylated Stat3 (Y705) (p-Stat3), Stat3, Bcl2 (Cell Signaling Technology, USA); Bax (EMD Millipore, USA), and β-tubulin (Abcam, USA).

Techniques: Western Blot, Quantitative RT-PCR, Expressing, Knockdown, Transfection, Control, Real-time Polymerase Chain Reaction, Gene Expression, Binding Assay, Sequencing, RNA Immunoprecipitation, Software